Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G58070.1
Common NameGIS, T10K17.280
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family C2H2
Protein Properties Length: 253aa    MW: 28456.8 Da    PI: 7.7141
Description C2H2 family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G58070.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
      zf-C2H2   1 ykCpdCgksFsrksnLkrHirtH 23 
                  ++C++C + F+++  L  H++ H
                  89******************988 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF576671.06E-588118No hitNo description
PfamPF139122.0E-590115IPR007087Zinc finger, C2H2
PROSITE profilePS5015710.9991118IPR007087Zinc finger, C2H2
PROSITE patternPS00028093113IPR007087Zinc finger, C2H2
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0007275Biological Processmulticellular organism development
GO:0009739Biological Processresponse to gibberellin
GO:0010091Biological Processtrichome branching
GO:0003676Molecular Functionnucleic acid binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008270Molecular Functionzinc ion binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000014anatomyrosette leaf
PO:0000037anatomyshoot apex
PO:0000112anatomyshoot axis epidermis
PO:0000229anatomyflower meristem
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009025anatomyvascular leaf
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
Sequence ? help Back to Top
Protein Sequence    Length: 253 aa     Download sequence    Send to blast
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G58070-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in inflorescence meristems, floral meristems and stem epidermis. {ECO:0000269|PubMed:16679458}.
Functional Description ? help Back to Top
Source Description
TAIRPutative transcription factor, contains C2H2 domain, regulates aspects of shoot maturation in Arabidopsis thaliana. GIS loss-of-function mutations affect the epidermal differentiation of inflorescence organs, causing a premature decrease in trichome production on successive leaves, stem internodes, and branches. Overexpression has the opposite effect on trichome initiation and causes other heterochronic phenotypes, affecting flowering and juvenileadult leaf transition and inducing the formation of rosette leaves on inflorescence stems.
UniProtProbable transcription factor required for the initiation of inflorescence trichomes in response to gibberellin (GA). Does not play a significant role in the cytokinin response. Acts upstream of the trichome initiation regulators GL1 and GL3, and downstream of the GA signaling repressor SPINDLY (SPY). Mediates the induction of GL1 expression by GA in inflorescence organs and is antagonized in its action by the DELLA repressor GAI. Controls trichome branching downstream of the key regulator STICHEL (STI) in an endoreduplication-independent pathway. {ECO:0000269|PubMed:16679458, ECO:0000269|PubMed:17507408, ECO:0000269|PubMed:22210898, ECO:0000269|PubMed:23825141}.
Function -- GeneRIF ? help Back to Top
  1. GLABROUS INFLORESCENCE STEMS (GIS) is a putative C2H2 transcription factor which regulates several aspects of shoot maturation in Arabidopsis.
    [PMID: 16679458]
  2. GIS is required for the gibberellic acid-regulated trichome branching. GIS is not required for cell endoreduplication but functions downstream of the key regulator STI to control trichome branching in an endoreduplication-independent pathway.
    [PMID: 22210898]
  3. show that ZFP5 encodes a protein functionally equivalent to GIS and GIS2 in controlling trichome initiation
    [PMID: 22301962]
  4. GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM
    [PMID: 23825141]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By gibberellin. {ECO:0000269|PubMed:16679458}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT2G01570 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT3G27920(A), AT5G41315(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Reduced trichome production on cauline leaves, stem internodes and branches, and sepals. {ECO:0000269|PubMed:16679458, ECO:0000269|PubMed:17507408, ECO:0000269|PubMed:22210898}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G58070
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAL1370810.0AL137081.1 Arabidopsis thaliana DNA chromosome 3, BAC clone F9D24.
GenBankBT0033400.0BT003340.1 Arabidopsis thaliana zinc finger-like protein (At3g58070) mRNA, complete cds.
GenBankBT0096760.0BT009676.1 Arabidopsis thaliana At3g58070 mRNA, complete cds.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_191366.10.0putative transcription factor GIS
SwissprotQ84WI00.0GIS_ARATH; Zinc finger protein GIS
TrEMBLD7LVZ71e-153D7LVZ7_ARALL; Putative uncharacterized protein
STRINGAT3G58070.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP285958
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  3. Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK
    Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.
    Plant J., 2004. 38(2): p. 366-79
  4. Englbrecht CC,Schoof H,B
    Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome.
    BMC Genomics, 2004. 5(1): p. 39
  5. Kiba T, et al.
    Combinatorial microarray analysis revealing arabidopsis genes implicated in cytokinin responses through the His->Asp Phosphorelay circuitry.
    Plant Cell Physiol., 2005. 46(2): p. 339-55
  6. Nagata T,Yamada H,Du Z,Todoriki S,Kikuchi S
    Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis.
    J. Agric. Food Chem., 2005. 53(4): p. 1022-30
  7. Gan Y, et al.
    GLABROUS INFLORESCENCE STEMS modulates the regulation by gibberellins of epidermal differentiation and shoot maturation in Arabidopsis.
    Plant Cell, 2006. 18(6): p. 1383-95
  8. Xin Z,Mandaokar A,Chen J,Last RL,Browse J
    Arabidopsis ESK1 encodes a novel regulator of freezing tolerance.
    Plant J., 2007. 49(5): p. 786-99
  9. Gan Y,Liu C,Yu H,Broun P
    Integration of cytokinin and gibberellin signalling by Arabidopsis transcription factors GIS, ZFP8 and GIS2 in the regulation of epidermal cell fate.
    Development, 2007. 134(11): p. 2073-81
  10. Gan Y,Yu H,Peng J,Broun P
    Genetic and molecular regulation by DELLA proteins of trichome development in Arabidopsis.
    Plant Physiol., 2007. 145(3): p. 1031-42
  11. Ciftci-Yilmaz S,Mittler R
    The zinc finger network of plants.
    Cell. Mol. Life Sci., 2008. 65(7-8): p. 1150-60
  12. Yu N, et al.
    Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana.
    Plant Cell, 2010. 22(7): p. 2322-35
  13. Zhou Z, et al.
    Zinc finger protein5 is required for the control of trichome initiation by acting upstream of zinc finger protein8 in Arabidopsis.
    Plant Physiol., 2011. 157(2): p. 673-82
  14. An L,Zhou Z,Su S,Yan A,Gan Y
    GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome branching through gibberellic acid signaling in Arabidopsis.
    Plant Cell Physiol., 2012. 53(2): p. 457-69
  15. Zhou Z,An L,Sun L,Gan Y
    ZFP5 encodes a functionally equivalent GIS protein to control trichome initiation.
    Plant Signal Behav, 2012. 7(1): p. 28-30
  16. Yan A,Pan J,An L,Gan Y,Feng H
    The responses of trichome mutants to enhanced ultraviolet-B radiation in Arabidopsis thaliana.
    J. Photochem. Photobiol. B, Biol., 2012. 113: p. 29-35
  17. Meinke DW
    A survey of dominant mutations in Arabidopsis thaliana.
    Trends Plant Sci., 2013. 18(2): p. 84-91
  18. Zhou Z, et al.
    Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana.
    New Phytol., 2013. 198(3): p. 699-708
  19. Sun LL, et al.
    GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis.
    J Zhejiang Univ Sci B, 2013. 14(7): p. 563-9
  20. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73